Preliminary Protocol Development of a HPLC-TBARS-EVSC (Ex Vivo Stratum Corneum) Assay for Skin Research: Application in a Sunscreen System

Considering the importance of cutaneous tissue investigation and need for development new protocols to non-invasively establish safety efficacy dermocosmetics topical products, we aimed at developing an HPLC-TBARS-EVSC (high performance liquid chromatography–thiobarbituric acid reactive species–ex vivo stratum corneum) assay lipid peroxidation measurement on subjects’ corneum (SC) obtained by tape stripping; additionally, applied in emulsified sunscreen system containing ethylhexyl triazone bemotrizinol as UV filters. HPLC analysis was performed isocratic mode with 35% methanol/65% phosphate buffer (pH 7.0) mobile phase. The diode detector set 532 nm quantify malondialdehyde (MDA)-TBA adduct. An ex stripping method 10 volunteers three pre-defined regions volar forearms: control; irradiated; site (2.0 mg·cm?2). Ten adhesive tapes per region were used SC removal. exclusive protocol measure preliminarily developed linearity selectivity. suggested use artificial irradiation dose (5506 KJ·m?2) improve response from SC. had a significative decrease lipoperoxidative damage compared control. Our can aid establishment anti-UV antioxidant agents, instance, studies that aim elucidating level even carrying out baseline investigations characterizing different ethnicities genders.